MLPA based techniques

The technology of Multiplex Ligation-Dependent Probe Amplification (MLPA) has enabled analysis of microdeletion/microduplication syndromes, subtelomeric chromosome aberrations and methylation quantification of genomic DNA. This technology is a variation of the multiplex PCR that permits simultaneous amplification of multiple targets. The MLPA reaction can be divided in five major steps: DNA denaturation and hybridisation of MLPA probes, ligation reaction, PCR reaction, separation of amplification products by capillary electrophoresis and data analysis with a software. The relative quantity of each amplicon can be determined by comparing the amplified DNA from a patient to a control sample. This can indicate the presence of DNA rearrangements.

More details at the link:   https://www.mrcholland.com/technology/mlpa/technique